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Related Experiment Video

Updated: Jul 7, 2026

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Published on: December 3, 2010

Profiling human gene expression with cDNA microarrays.

Y Jiang¹, J Lueders, A Glatfelter

¹National Human Genome Research Institute, NIH, Bethesda, Maryland, USA.

Current Protocols in Molecular Biology

|February 12, 2008

Summary

This study presents a method for analyzing gene expression using complementary DNA (cDNA) clones. These techniques enable parallel gene expression assays by utilizing cDNA microarrays for hybridization detection.

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Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Genomics initiatives have increased the availability of complementary DNA (cDNA) clones representing gene 3' ends.
These cDNA clones can serve as valuable tools for gene expression analysis.

Purpose of the Study:

To describe methods for utilizing 3'-end human gene cDNA clones in gene expression assays.
To enable highly parallel analysis of gene expression.

Main Methods:

Preparation of cDNA microarrays.
Extraction of RNA from cells.
Preparation of fluorescently labeled cDNA from RNA pools.
Hybridization of labeled cDNA to microarrays.

Main Results:

The described methods allow for the use of cDNA clones as hybridization detectors.
A highly parallel assay for gene expression is achievable.

Conclusions:

The protocols facilitate the application of readily available cDNA clones for comprehensive gene expression profiling.
This approach offers an efficient method for large-scale gene expression studies.