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Updated: Jul 7, 2026

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Published on: May 29, 2018

Chimeric mouse production by microinjection.

¹Harvard Medical School, Boston, Massachusetts, USA.

Current Protocols in Molecular Biology

|February 12, 2008

Summary

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Generating genetically modified mouse models is crucial for research. This study details methods for creating chimeric mice from embryonic stem cells, enabling the propagation of specific mutations for future generations.

Area of Science:

Genetics
Developmental Biology
Animal Models

Background:

Mouse embryonic stem (ES) cells are instrumental in creating genetically engineered mouse models.
Homologous recombination in ES cells combined with blastocyst-mediated transgenesis allows for the generation of tailored mutant mouse strains.
These mutant mice serve as invaluable research tools, offering capabilities not achievable through other methods.

Purpose of the Study:

To describe the detailed methodology for generating chimeric mice.
To outline the process from ES cell injection into blastocysts to embryo implantation in foster mothers.
To explain how chimeras can be used to propagate mutations through the germ line.

Main Methods:

Injection of mouse embryonic stem (ES) cells into the blastocoel cavity of 3.5-day-old embryos.

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Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity

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Related Experiment Videos

Last Updated: Jul 7, 2026

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Published on: May 29, 2018

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity

Published on: June 25, 2015

Implantation of injected embryos into a foster mother for gestation.

Identification and breeding of chimeric offspring.

Main Results:

Successful generation of chimeric mice with tissues derived from both host embryo and ES cells.
Demonstration of the potential for ES cells to colonize the germ line within the chimera.
Establishment of a method to produce new mouse strains carrying specific genetic alterations.

Conclusions:

The described methods provide a robust protocol for generating chimeric mice.
Chimeric mice are essential for creating genetically modified mouse lines for research.
This technique facilitates the propagation of targeted mutations, advancing genetic research and disease modeling.