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Differential display of mRNA by PCR

P Liang1, A B Pardee

  • 1Vanderbilt-Ingram Cancer Center, Nashville, Tennessee, USA.

Current Protocols in Molecular Biology
|February 12, 2008
PubMed

Abstract:

Formerly UNIT , this unit describes how differential display techniques allow the identification and subsequent isolation of differentially expressed genes that requires no knowledge of sequences, but rather PCR amplification using arbitrary oligonucleotides and high resolution polyacrylamide gel electrophoresis.

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