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Updated: Jul 7, 2026

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Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR
Published on: February 6, 2026
Differential display of mRNA by PCR
1Vanderbilt-Ingram Cancer Center, Nashville, Tennessee, USA.
Current Protocols in Molecular Biology
|February 12, 2008
Abstract:
Formerly UNIT , this unit describes how differential display techniques allow the identification and subsequent isolation of differentially expressed genes that requires no knowledge of sequences, but rather PCR amplification using arbitrary oligonucleotides and high resolution polyacrylamide gel electrophoresis.
Related Concept Videos
Real Time RT-PCR
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
PCR - Polymerase Chain Reaction
Overview

