Related Experiment Video

Updated: Jul 7, 2026

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR
07:06

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR

Published on: February 6, 2026

Differential display of mRNA by PCR

P Liang1, A B Pardee

  • 1Vanderbilt-Ingram Cancer Center, Nashville, Tennessee, USA.

Current Protocols in Molecular Biology
|February 12, 2008
PubMed

Abstract:

Formerly UNIT , this unit describes how differential display techniques allow the identification and subsequent isolation of differentially expressed genes that requires no knowledge of sequences, but rather PCR amplification using arbitrary oligonucleotides and high resolution polyacrylamide gel electrophoresis.

More Related Videos

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
07:26

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Published on: July 29, 2019

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

Related Experiment Videos

Last Updated: Jul 7, 2026

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR
07:06

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR

Published on: February 6, 2026

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
07:26

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Published on: July 29, 2019

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

Overview

Articles linked to this work by shared authors, journal, and citation graph.

[The value of spectral CT-based radiomics in preoperative prediction of lymph node metastasis of advanced gastric cancer].

Zhonghua yi xue za zhi·2020

Mapping the Brain's electric fields with Magnetoelectric nanoparticles.

Bioelectronic medicine·2020

Discovery of an Aedes (Stegomyia) albopictus population and first records of Aedes (Stegomyia) aegypti in Canada.

Medical and veterinary entomology·2019

[A functional magnetic resonance imaging study of exercise addiction].

Zhonghua yi xue za zhi·2019

[Relationship between one-stop CT spectral perfusion imaging parameters and expression of lymphatic microvessel density and vascular endothelial growth factor-C in axillary lymph nodes of rabbit VX2 breast cancer].

Zhonghua yi xue za zhi·2019

[Application of a multi-material artifact reduction algorithm in a wide-detector CT in the evaluation of the portal venous angiography of postoperative TIPS and embolization].

Zhonghua yi xue za zhi·2019

Laccase Immobilization on Tannic Acid-Coated Nylon Membrane for Dye Decolorization.

ACS omega·2026

Concentration of volatile organic compounds within indoor dairy facilities.

Journal of environmental quality·2026

Interfacial dual regulation of charge density and structural accessibility for efficient electrodialytic lithium extraction.

Water research·2026

Prediction of lignocellulosic pretreatment performance using deep eutectic solvents based on IWOA-Stacking.

Bioresource technology·2026

Impact of sialyltransferase deletion in mature T cells on control of subcutaneous and metastatic cancers.

Glycobiology·2026

Machine Learning-Enhanced Three-Channel Cu-Pt Bimetallic Nanozyme Sensor Array for Multi-Class Pesticide Fingerprint Discrimination.

ACS applied materials & interfaces·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us