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Mapping Mammalian 3D Genome Interactions with Micro-C-XL
Published on: November 3, 2023
Mapping by multiple endonuclease digestions
1Harvard Medical School, Boston, Massachusetts, USA.
Current Protocols in Molecular Biology
|February 12, 2008
Abstract:
In this unit, a protocol is provided in which the DNA of interest is cleaved with a variety of restriction endonucleases, either individually or in combination, and the resulting products are separated by agarose or acrylamide gel electrophoresis. By determining the sizes of DNA fragments produced by endonuclease cleavage, the restriction map is deduced progressively from simple situations where enzymes cleave the DNA once or twice to more complex situations where cleavage occurs more frequently.
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