Amplification of cosmid and plasmid libraries

The purpose of amplification is to provide a reagent library that can be used many times. In this protocol, bacteria containing the recombinant clones are grown on agar plates, washed off the plates, and stored in glycerol. An amplified cosmid or plasmid library will contain one library's equivalence in 10 to 100 microl. By producing 100 microl of such a library frozen in 100 1-ml tubes, over 1000 platings can be expected.