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Updated: Jul 7, 2026

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
Published on: March 26, 2018
Production of a complete cDNA library
1Brigham and Women's Hospital, Boston, Massachusetts, USA.
Abstract:
A complete cDNA library is one that contains at least one cDNA clone representing each mRNA in the cell. A method is provided in this unit for calculating the required base of a cDNA library such that the library should contain at least one copy of every mRNA. Either a phage or plasmid vector can be used in constructing a complete cDNA library, and protocols are described for both. Procedures for evaluating these cDNA libraries are also provided along with a procedure for evaluating the quality of the lambda arms used in constructing complete phage cDNA libraries.
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