Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA sequencing by the dideoxy method.

B E Slatko1, L M Albright, S Tabor

  • 1New England Biolabs, Beverly, Massachusetts, USA.

Current Protocols in Molecular Biology
|February 12, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Black Hole Spectroscopy and Tests of General Relativity with GW250114.

Physical review letters·2026
Same author

[Glottic closure to prevent aspiration following supraglottic horizontal partial laryngectomy in elderly patients].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2025
Same author

[The correlations between clinical characteristics including lesion site of papillary thyroid carcinoma and cervical lymph node metastasis].

Zhonghua zhong liu za zhi [Chinese journal of oncology]·2025
Same author

Periodontitis Promotes OSCC and Prostate Cancer Progression and Immunosuppression by Skewing Myeloid Differentiation of HSPCs.

Journal of dental research·2025
Same author

GW250114: Testing Hawking's Area Law and the Kerr Nature of Black Holes.

Physical review letters·2025
Same author

[Analysis on the current status and characteristic of clinical trials for oral diseases in China].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2025
Same journal

Nondenaturing Polyacrylamide Gel Electrophoresis: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Purification and Concentration of DNA from Aqueous Solutions: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Expression of Proteins Using Semliki Forest Virus Vectors: Protein Expression.

Current protocols in molecular biology·2021
Same journal

Methylation and Uracil Interference Assays for Analysis of Protein-DNA Interactions: DNA-Protein Interactions.

Current protocols in molecular biology·2021
Same journal

Separation of Double- and Single-Stranded Nucleic Acids Using Hydroxylapatite Chromatography: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Pulsed-Field Gel Electrophoresis: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
See all related articles

Dideoxy sequencing methods, including Sanger and thermal cycle sequencing, enable DNA sequence determination using DNA polymerase and dideoxyribonucleoside triphosphates (ddNTPs) for chain termination. These techniques are crucial for genetic analysis.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • DNA sequencing is fundamental to molecular biology.
  • The dideoxy chain termination method, pioneered by Sanger, revolutionized DNA analysis.
  • Understanding variations in sequencing protocols is key to advancing genetic research.

Purpose of the Study:

  • To describe the fundamental dideoxy sequencing reaction.
  • To present three variations of the dideoxy sequencing procedure: labeling/termination, Sanger, and thermal cycle sequencing.
  • To detail the application of automated fluorescent sequencers in DNA sequencing.

Main Methods:

  • DNA polymerase extends a primer annealed to a DNA template.
  • Deoxyribonucleoside triphosphates (dNTPs) and dideoxyribonucleoside triphosphates (ddNTPs) are used.

Related Experiment Videos

  • Incorporation of ddNTPs terminates DNA elongation, visualized via gel electrophoresis and autoradiography.

    • Main Results:

    • Three distinct dideoxy sequencing protocols are detailed.
    • Thermal cycle sequencing offers advantages like reduced template DNA requirements and streamlined steps.
    • Automated fluorescent sequencing systems provide efficient, multi-color DNA analysis.

    Conclusions:

    • Dideoxy sequencing, in its various forms, remains a vital tool for DNA sequence determination.
    • Thermal cycle sequencing enhances efficiency and reduces sample input.
    • Automated sequencing technologies facilitate high-throughput genetic analysis.