Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Two-dimensional Gel Electrophoresis

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

SDS-PAGE

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...

DNA Agarose Gel Electrophoresis

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

First-in-Human Study of a Novel Coronary Crossing Device for Calcified Lesions.

JACC. Cardiovascular interventions·2025

Same author

Analysis of seat belt buckle release forces in post-rollover scenarios: Implications for passenger safety.

Applied ergonomics·2025

Same author

A fatigue failure framework for the assessment of highly variable low back loading using inertial motion capture - a case study.

Ergonomics·2025

Same author

Critical review of chronic toxicity testing approaches with the saltwater mysid (Americamysis bahia) used in pesticide registration.

Environmental toxicology and chemistry·2025

Same author

Scan/rescan reliability of magnetic resonance imaging (MRI).

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2025

Same author

Expedited conveyance of out-of-hospital-cardiac arrest patients with STEMI and shockable rhythms to Cardiac Arrest Centres - A feasibility pilot study of the British Cardiovascular Intervention Society conveyance algorithm.

Resuscitation·2025

Search research articles

Related Experiment Video

Updated: Jul 7, 2026

Electroeluting DNA Fragments

Electroeluting DNA Fragments

Published on: September 5, 2010

Overview of digital electrophoresis analysis.

Scott Medberry¹, Sean Gallagher, Butch Moomaw

¹Amersham Pharmacia Biotech, San Francisco, California, USA.

Current Protocols in Molecular Biology

|February 12, 2008

Summary

Digital gel electrophoresis documentation offers significant advantages over traditional photography for quantitation and analysis. This method enables rapid image capture and sophisticated data manipulation using specialized software.

More Related Videos

Denaturing Gradient Gel Electrophoresis (DGGE)

Denaturing Gradient Gel Electrophoresis (DGGE)

Published on: February 25, 2007

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

Related Experiment Videos

Last Updated: Jul 7, 2026

Electroeluting DNA Fragments

Electroeluting DNA Fragments

Published on: September 5, 2010

Denaturing Gradient Gel Electrophoresis (DGGE)

Denaturing Gradient Gel Electrophoresis (DGGE)

Published on: February 25, 2007

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

Area of Science:

Molecular Biology
Biotechnology
Biochemistry

Background:

Gel electrophoresis is a fundamental technique for biomolecule separation in molecular biology.
Traditional documentation relies on silver halide photography, suitable for qualitative analysis.
Digital imaging presents opportunities for enhanced quantitation and sophisticated analysis.

Purpose of the Study:

To present the advantages of digital documentation and analysis for gel electrophoresis.
To define key terms related to gel imaging.
To review image capture devices and digital analysis methods.

Main Methods:

Digital image capture using specialized devices.
Image manipulation and analysis using software.
Overview of one- and two-dimensional digital gel analysis techniques.

Main Results:

Digital capture allows rapid acquisition of gel electropherogram images.
Image analysis software facilitates sophisticated data manipulation and quantitation.
Digital methods provide significant advantages for quantitative gel documentation.

Conclusions:

Digital documentation and analysis are superior to traditional methods for quantitative gel electrophoresis.
The adoption of digital imaging enhances the utility of gel electrophoresis in research.
This approach supports advanced analysis of biomolecular separation data.