Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunoblotting and immunodetection.

Sean Gallagher1, Scott E Winston, Steven A Fuller

  • 1UVP, Inc., Upland, California, USA.

Current Protocols in Molecular Biology
|February 12, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First-in-Human Study of a Novel Coronary Crossing Device for Calcified Lesions.

JACC. Cardiovascular interventions·2025
Same author

Analysis of seat belt buckle release forces in post-rollover scenarios: Implications for passenger safety.

Applied ergonomics·2025
Same author

A fatigue failure framework for the assessment of highly variable low back loading using inertial motion capture - a case study.

Ergonomics·2025
Same author

Critical review of chronic toxicity testing approaches with the saltwater mysid (Americamysis bahia) used in pesticide registration.

Environmental toxicology and chemistry·2025
Same author

Scan/rescan reliability of magnetic resonance imaging (MRI).

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2025
Same author

Expedited conveyance of out-of-hospital-cardiac arrest patients with STEMI and shockable rhythms to Cardiac Arrest Centres - A feasibility pilot study of the British Cardiovascular Intervention Society conveyance algorithm.

Resuscitation·2025
Same journal

Nondenaturing Polyacrylamide Gel Electrophoresis: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Purification and Concentration of DNA from Aqueous Solutions: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Expression of Proteins Using Semliki Forest Virus Vectors: Protein Expression.

Current protocols in molecular biology·2021
Same journal

Methylation and Uracil Interference Assays for Analysis of Protein-DNA Interactions: DNA-Protein Interactions.

Current protocols in molecular biology·2021
Same journal

Separation of Double- and Single-Stranded Nucleic Acids Using Hydroxylapatite Chromatography: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Pulsed-Field Gel Electrophoresis: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
See all related articles

Western blotting is a key technique for identifying specific antigens using antibodies. This method involves protein solubilization, separation, membrane transfer, and antibody probing for detection.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Immunoblotting, commonly known as western blotting, is a widely used technique.
  • It enables the specific identification of antigens using antibodies.

Purpose of the Study:

  • To provide comprehensive protocols for western blotting.
  • To detail each step from protein sample preparation to final detection.

Main Methods:

  • Protein sample solubilization using SDS and reducing agents.
  • Separation of proteins via SDS-PAGE.
  • Electrophoretic transfer of separated proteins to a membrane.
  • Immunodetection using primary and secondary antibodies.
  • Detection via enzyme-conjugated antibodies and substrates.

Related Experiment Videos

Main Results:

  • Successful transfer and binding of proteins to the membrane.
  • Specific detection of target antigens via antibody-antigen complex formation.
  • Visualization of results using chromogenic or luminescent substrates.

Conclusions:

  • Western blotting is a robust method for antigen identification.
  • The described protocols ensure efficient and reliable execution of the technique.
  • Membranes can be stripped and reprobed for further analysis.