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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Transgenic Organisms

Transgenic Organisms

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Related Experiment Video

Updated: Jul 7, 2026

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

Published on: June 24, 2022

Transgenic mouse production by zygote injection.

David A Conner¹

¹Harvard Medical School, Boston, Massachusetts, USA.

Current Protocols in Molecular Biology

|February 12, 2008

Summary

This study details creating transgenic mice by injecting DNA into fertilized eggs. It covers egg preparation, DNA injection, and reimplantation for genetic modification research.

Area of Science:

Genetics and Molecular Biology
Developmental Biology
Animal Models

Background:

Transgenic mice are crucial models for studying gene function and disease.
Efficient methods for generating transgenic mice are essential for biomedical research.

Purpose of the Study:

To describe standardized methods for producing transgenic mice via DNA microinjection.
To outline the preparation of DNA constructs for efficient zygote microinjection.

Main Methods:

Isolation of fertilized mouse oocytes (zygotes).
Microinjection of plasmid or BAC DNA into zygotes.
Surgical reimplantation of injected zygotes into surrogate mothers.
Preparation of high-quality plasmid and BAC DNA for microinjection.

More Related Videos

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Published on: October 7, 2018

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Related Experiment Videos

Last Updated: Jul 7, 2026

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

Published on: June 24, 2022

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Published on: October 7, 2018

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Main Results:

Successful generation of transgenic mice using the described microinjection technique.
Demonstration of methods for preparing DNA suitable for microinjection.

Conclusions:

The described methods provide a robust protocol for transgenic mouse production.
These techniques facilitate the generation of genetically modified mouse models for research.