Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Transgenic Organisms

Transgenic Organisms

Transgenic Organisms

Transgenic Organisms

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

<i>ROBO2</i> Variants Associated With Atrial Septal Defect Define a Novel Regulatory Element.

Circulation. Genomic and precision medicine·2026

Same author

Efficient in vivo genome editing prevents hypertrophic cardiomyopathy in mice.

Nature medicine·2023

Same author

Ablation of lysophosphatidic acid receptor 1 attenuates hypertrophic cardiomyopathy in a mouse model.

Proceedings of the National Academy of Sciences of the United States of America·2022

Same author

<i>LAMP2</i> Cardiomyopathy: Consequences of Impaired Autophagy in the Heart.

Journal of the American Heart Association·2021

Same author

BET bromodomain proteins regulate transcriptional reprogramming in genetic dilated cardiomyopathy.

JCI insight·2020

Same author

CRISPR/Cas9-Mediated Fluorescent Tagging of Endogenous Proteins in Human Pluripotent Stem Cells.

Current protocols in human genetics·2018

Search research articles

Related Experiment Video

Updated: Jul 7, 2026

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Published on: October 7, 2018

Transgenic mouse colony management.

David A Conner¹

¹Harvard Medical School, Cambridge, Massachusetts, USA.

Current Protocols in Molecular Biology

|February 12, 2008

Summary

This summary is machine-generated.

This guide details establishing a transgenic mouse colony, covering essential mating and genotyping techniques for creating genetically modified animal models.

More Related Videos

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells

Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells

Published on: November 11, 2015

Related Experiment Videos

Last Updated: Jul 7, 2026

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Published on: October 7, 2018

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells

Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells

Published on: November 11, 2015

Area of Science:

Animal models
Genetics
Molecular biology

Background:

Transgenic mice are crucial for studying gene function and disease.
Establishing a stable transgenic colony requires specific breeding and identification protocols.

Purpose of the Study:

To outline practical strategies for establishing a transgenic mouse colony.
To introduce fundamental mating and genotyping methods for genetic modification.

Main Methods:

Description of basic breeding schemes for transgenic mouse lines.
Explanation of DNA extraction and PCR-based genotyping techniques.
Guidance on colony management and record-keeping.

Main Results:

Successful establishment of a transgenic mouse colony.
Accurate identification of transgenic animals through genotyping.
Efficient propagation of desired genetic modifications.

Conclusions:

Standardized protocols facilitate the reliable creation of transgenic mouse colonies.
Effective mating and genotyping are essential for genetic research using mouse models.