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Updated: Jul 7, 2026
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Isolation and Activation of Murine Lymphocytes
Published on: October 30, 2016
Isolation and immortalization of lymphocytes.
1Baylor College of Medicine, Houston, Texas, USA.
Current Protocols in Molecular Biology
|February 12, 2008
Summary
Generating lymphoblastoid cell lines (LCLs) is crucial for genomics research. This study details simple, effective procedures for producing EBV, isolating lymphocytes, and establishing LCLs for stable DNA and cell source.
Area of Science:
- Genomics
- Proteomics
- Cell Biology
- Virology
Background:
- Genomics and Proteomics advancements require a consistent supply of DNA from specific genotypes.
- Lymphoblastoid cell lines (LCLs) provide a stable, unlimited source of genomic DNA and viable cells for molecular studies.
Purpose of the Study:
- To describe simple procedures for the production and isolation of EBV-infected lymphoblastoid cell lines (LCLs).
- To provide a reliable method for generating LCLs for use in biochemical and molecular studies.
Main Methods:
- Production of Epstein-Barr virus (EBV).
- Isolation of primary lymphocytes.
- Infection of lymphocytes with EBV.
- Isolation and culture of resulting LCLs.
Main Results:
- Successful establishment of LCLs through EBV infection of primary lymphocytes.
- Demonstration of simple, reproducible procedures for LCL generation.
Conclusions:
- The described methods provide a straightforward approach to generating LCLs.
- LCLs are a valuable resource for ongoing genomic and proteomic research requiring stable DNA sources.
