Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Transduction

Transduction

Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Enhancement of RecET-mediated in vivo linear DNA assembly by a xonA mutation.

PloS one·2026

Same author

A λ Phage Platform for Successful Therapeutic Display of Protein Antigens.

bioRxiv : the preprint server for biology·2026

Same author

Correction: Recombineering: Genetic Engineering in Escherichia coli Using Homologous Recombination.

Current protocols·2024

Same author

Stability and gene strand bias of lambda prophages and chromosome organization in <i>Escherichia coli</i>.

mBio·2024

Same author

The crystal structure of bacteriophage λ RexA provides novel insights into the DNA binding properties of Rex-like phage exclusion proteins.

Nucleic acids research·2024

Same author

Everything OLD is new again: How structural, functional, and bioinformatic advances have redefined a neglected nuclease family.

Molecular microbiology·2023

Search research articles

Related Experiment Video

Updated: Jul 7, 2026

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes

Published on: September 1, 2018

E. coli genome manipulation by P1 transduction.

Lynn C Thomason¹, Nina Costantino¹, Donald L Court¹

¹National Cancer Institute at Frederick, Frederick, Maryland.

Current Protocols in Molecular Biology

|February 12, 2008

Summary

This summary is machine-generated.

This study details using P1 bacteriophage to transfer large E. coli genome fragments between strains. This method facilitates genetic engineering by moving DNA segments for research purposes.

More Related Videos

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Published on: October 31, 2014

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

Related Experiment Videos

Last Updated: Jul 7, 2026

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes

Published on: September 1, 2018

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Published on: October 31, 2014

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

Area of Science:

Microbiology
Molecular Biology
Genetics

Background:

Bacterial genome manipulation is crucial for understanding gene function.
Efficient methods for transferring large DNA fragments are needed in microbial genetics.

Purpose of the Study:

To describe a reliable method for transferring large E. coli genomic fragments between strains.
To outline the use of P1 bacteriophage for targeted genome modification.

Main Methods:

Utilizing P1 bacteriophage to package and transfer approximately 100 kb DNA fragments from a donor E. coli strain.
Infecting a recipient E. coli strain with a P1 phage lysate containing donor DNA.
Leveraging recipient strain's enzymatic machinery for homologous recombination to integrate transferred fragments.

Main Results:

Successful transfer of large E. coli genomic fragments (approx. 100 kb) mediated by P1 bacteriophage.
Demonstration of P1 bacteriophage's capability to act as a vector for bacterial DNA.
Integration of transferred DNA fragments into the recipient chromosome via genetic recombination.

Conclusions:

P1 bacteriophage-mediated transduction is an effective technique for large-scale genome fragment transfer in E. coli.
This method provides a valuable tool for constructing specific genetic variants and studying gene organization.
The described procedure enhances capabilities in bacterial genetics and synthetic biology.