Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 7, 2026

Facial Vein Venipuncture for Murine Blood Collection
05:01

Facial Vein Venipuncture for Murine Blood Collection

Published on: September 26, 2025

Tissue collection for systematic phenotyping in the mouse.

Cristina Antal1, Marius Teletin, Olivia Wendling

  • 1Institut Clinique de la Souris, Illkirch Cedex, France.

Current Protocols in Molecular Biology
|February 12, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Temperature determines early-life success of burbot Lota lota eggs.

Journal of fish biology·2026
Same author

Western diet-induced MASH in PWK/PhJ mice identifies disruptions in amino acid and sphingolipid metabolism contributing to cardiac dysfunction.

Nature communications·2026
Same author

Endometrial NCOR1 deficiency contributes to implantation failure in endometriosis-associated infertility.

iScience·2026
Same author

The PM20D1-OLE pathway induces microglia rewiring to ameliorate Alzheimer disease.

Cell death & disease·2026
Same author

Dynamics of genetic and somatic trade-offs in ageing and mortality.

Nature·2026
Same author

How to train your rodent: Recommendations for the preclinical study of exercise-induced benefits in metabolic research.

Cell metabolism·2026

This guide details mouse necropsy and tissue collection for anatomical, histological, or molecular studies. Proper tissue preservation is crucial, requiring different methods—fixation for histology and freezing for molecular analysis—to maintain sample integrity.

Area of Science:

  • Veterinary Pathology
  • Molecular Biology
  • Histology

Background:

  • Accurate post-mortem analysis in mice is essential for research.
  • Tissue preservation is critical for both anatomical defect assessment and molecular applications.
  • Current methods for tissue preservation present challenges when aiming for simultaneous histological and molecular analyses.

Purpose of the Study:

  • To provide a standardized procedure for mouse post-mortem examination and tissue collection.
  • To outline distinct tissue preservation techniques for necropsy, histology, and molecular biology.
  • To highlight the impact of simultaneous procedures on sample quality.

Main Methods:

  • Detailed protocol for gross post-mortem examination (necropsy).
  • Procedures for tissue collection for histological analysis, emphasizing rapid fixation.

More Related Videos

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
09:37

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

Mouse Adipose Tissue Collection and Processing for RNA Analysis
11:13

Mouse Adipose Tissue Collection and Processing for RNA Analysis

Published on: January 31, 2018

Related Experiment Videos

Last Updated: Jul 7, 2026

Facial Vein Venipuncture for Murine Blood Collection
05:01

Facial Vein Venipuncture for Murine Blood Collection

Published on: September 26, 2025

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
09:37

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

Mouse Adipose Tissue Collection and Processing for RNA Analysis
11:13

Mouse Adipose Tissue Collection and Processing for RNA Analysis

Published on: January 31, 2018

  • Protocols for tissue collection for molecular biology applications, emphasizing rapid freezing to preserve mRNA integrity.

    • Main Results:

    • Established distinct preservation methods tailored to specific analytical goals.
    • Demonstrated that rapid fixation is optimal for histology.
    • Demonstrated that rapid freezing is optimal for preserving mRNA for molecular studies.

    Conclusions:

    • Simultaneous tissue preservation for both histological and molecular analyses can compromise sample quality.
    • Researchers must choose preservation methods based on their primary objective.
    • Optimized protocols are provided for maximizing tissue integrity for specific downstream applications.