Related Experiment Video
Updated: Jul 7, 2026

A Method for Adapting Euglena gracilis to Cassava-Based Substrates for Heterotrophic Cultivation
Published on: June 12, 2026
Modification of amino acid and sugar transport in uncoupler-adapted Euglena gracilis
1Department of Biochemistry, North Carolina State University, Raleigh, North Carolina 27607, USA.
Abstract:
Uncoupler-adapted Euglena gracilis have a greatly impaired capacity to take up and incorporate some exogenously supplied amino acids and sugars. The degree of inhibition varies widely from >90% in the case of valine or glucose to none in the case of histidine. The inhibition is due to a decreased activity of the transport mechanism itself and is not due to either a lesion in the control mechanism for endogenous amino acid or sugar synthesis nor to a direct inhibition of the transport mechanism by uncouplers. No preferential labeling of mitochondrial membranes by [14C]amino acids occurs during the process of adaptation, a time when no cell division occurs. Apparently, during the long time required for adaptation, there occurs no major modification of mitochondrial proteins which could explain the subsequent resistance to uncouplers.
More Related Videos
13:16Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
Published on: December 31, 2019
05:47Optimizing Visualization of Axonal Transport of Endogenous Cargo by Fluorescence Microscopy in Living Caenorhabditis elegans
Published on: February 16, 2024
Related Concept Videos
Secondary Active Transport
Secondary Active Transport
Evolution of New Traits in Microbes
Diversity of Protists I
Glucose Transporters
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Membrane Proteins