Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Diffusion01:12

Diffusion

Diffusion is the passive movement of substances down their concentration gradients—requiring no expenditure of cellular energy. Substances, such as molecules or ions, diffuse from an area of high concentration to an area of low concentration in the cytosol or across membranes. Eventually, the concentration will even out, with the substance moving randomly but causing no net change in concentration. Such a state is called dynamic equilibrium, which is essential for maintaining overall...
Diffusion01:21

Diffusion

Diffusion is a type of passive transport. In passive transport, a substance tends to move from an area of high concentration to an area of low concentration until the concentration is equal across the space. For example, take the diffusion of substances through the air. When someone opens a perfume bottle in a room filled with people, the perfume is at its highest concentration in the bottle and is at its lowest at the edges of the room. The perfume vapor will diffuse, or spread away, from the...
Passive Diffusion: Overview and Kinetics01:17

Passive Diffusion: Overview and Kinetics

Passive diffusion is a critical process that allows small lipophilic drugs to cross the cell membrane along a concentration gradient. This mechanism's efficiency depends on four primary factors: the membrane's surface area, the drug's lipid-water partition coefficient, the concentration gradient, and the membrane's thickness.
When administered orally, drugs establish a substantial concentration gradient between the gastrointestinal (GI) lumen and the bloodstream, expediting their diffusion into...
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
Facilitated Diffusion01:16

Facilitated Diffusion

The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
External and Internal Respiration01:24

External and Internal Respiration

External respiration occurs in the lungs, and it is the first step in the journey of oxygen inside the body. When we inhale, oxygen enters our lungs and diffuses across the thin alveolar membrane. The alveoli are tiny, air-filled sacs that provide a vast surface area for gas exchange. Oxygen in the alveoli has a higher partial pressure (105 mmHg) than in the adjacent pulmonary capillaries (40 mmHg), establishing a pressure gradient. As a result, oxygen molecules move from the alveoli into the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The role of sulfur vacancies on FeS<sub>2</sub>(100) in NO dissociative adsorption: a combined <i>in situ</i> SR-XPS and DFT calculation study.

Physical chemistry chemical physics : PCCP·2026
Same author

Operating Room Extubation in Major Aortic Surgery: Policy Change Leading to Improved Recovery.

Journal of cardiothoracic and vascular anesthesia·2026
Same author

Right Anterior Thoracotomy Versus Partial Sternotomy for Isolated Aortic Valve Replacement: A Propensity Analysis of Clinical Outcomes and Hospital Costs.

Medicina (Kaunas, Lithuania)·2026
Same author

Thermodynamics and Kinetics of Two-Dimensional H<sub>2</sub> Gas on Ag(111) Studied by Tip-Enhanced Raman Spectroscopy.

Nano letters·2026
Same author

Surface-Enhanced Infrared Absorption on Arranged Pt Nanoantennas.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same author

Robotic Left Atrial Appendage Occlusion: Insights From Real-World Practice.

The international journal of medical robotics + computer assisted surgery : MRCAS·2026

Related Experiment Video

Updated: Jul 7, 2026

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

Microscopic diffusion processes of NO on the Pt997 surface.

Noriyuki Tsukahara1, Kozo Mukai, Yoshiyuki Yamashita

  • 1The Institute for Solid State Physics, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581, Japan.

The Journal of Chemical Physics
|February 13, 2008
PubMed
Summary

Nitric oxide (NO) molecules were observed to migrate across platinum surfaces at low temperatures. Researchers mapped NO diffusion pathways and energy barriers on Pt(997), revealing specific adsorption sites and migration dynamics.

More Related Videos

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics
07:57

Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics

Published on: November 10, 2014

Related Experiment Videos

Last Updated: Jul 7, 2026

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics
07:57

Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics

Published on: November 10, 2014

Area of Science:

  • Surface Science
  • Physical Chemistry
  • Materials Science

Background:

  • Understanding molecular diffusion on surfaces is crucial for catalysis and materials design.
  • The Pt(997) surface offers diverse adsorption sites, including terraces and step edges, influencing diffusion behavior.
  • Low-coverage NO adsorption on platinum surfaces presents a model system for studying fundamental surface dynamics.

Purpose of the Study:

  • To investigate the microscopic diffusion processes of nitric oxide (NO) molecules on the Pt(997) surface at low coverage.
  • To identify and characterize various adsorption sites for NO on Pt(997) at cryogenic temperatures.
  • To quantify the migration barriers and kinetics of NO diffusion between different adsorption sites.

Main Methods:

  • Time-resolved infrared reflection absorption spectroscopy (TR-IRAS) was employed to monitor NO adsorption and diffusion.
  • Kinetic Monte Carlo (KMC) simulations were used in conjunction with experimental data.
  • Experiments were conducted at various low temperatures (11 K to 110 K) to probe different diffusion regimes.

Main Results:

  • Four distinct adsorption sites (OT, HT, BS, HS) for NO on Pt(997) were identified at 11 K, with a mean lateral displacement of 4.1 Å.
  • Migration of hollow sites at the step (HS) to bridge sites at the step (BS) was observed with a barrier of ~120 meV.
  • Diffusion from on-top (OT) to hollow terrace (HT) sites occurred with an activation barrier of 200 meV, while HT to BS migration had a barrier of 290 meV.

Conclusions:

  • A quantitative microscopic picture of NO migration on the Pt(997) surface has been established.
  • The study provides detailed insights into the energetic landscape governing NO diffusion on stepped platinum surfaces.
  • The findings contribute to a fundamental understanding of surface processes relevant to heterogeneous catalysis.