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Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Chromatin Structure and RNA Splicing

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Related Experiment Video

Updated: Jul 7, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Extent and diversity of human alternative splicing established by complementary database annotation and microarray

Jonathan L Bingham¹, Patricia E Carrigan, Laurence J Miller

¹Jivan Biologics, Inc., Larkspur, California 94939, USA.

Omics : a Journal of Integrative Biology

|February 13, 2008

Summary

This summary is machine-generated.

Alternative splicing in humans is more complex than previously thought, involving more than just exon skipping. This study reveals the diverse landscape of splicing events and their differential expression across cell types.

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

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Area of Science:

Molecular Biology
Genomics
Bioinformatics

Background:

Alternative splicing generates functional diversity in higher organisms.
Previous studies focused mainly on exon-skip events, neglecting other splice types.
The prevalence and importance of diverse alternative splicing events remain largely unexplored.

Purpose of the Study:

To comprehensively measure and analyze differential expression of various alternative splicing types in human cells.
To assess the complexity of alternative splicing beyond canonical exon-skip events.
To validate a new human splice variant database and genome-wide microarray approach.

Main Methods:

Utilized a novel human splice variant database for genome-wide annotation.
Employed a genome-wide microarray to probe thousands of splice events across multiple types.
Measured differential splicing event expression across six diverse human cell line pairs.

Main Results:

Alternative splicing in humans is significantly more complex than previously characterized.
Exon-skip events represent a minority of observed splicing differences.
The frequency of differential expression aligns with annotation findings, highlighting diverse splice event roles.

Conclusions:

Alternative splicing in human cells exhibits greater complexity than the commonly cited exon-skip mechanism.
Genome-wide annotation and high-throughput splicing microarrays offer powerful tools for studying alternative splicing.
This research provides a foundation for understanding the role of diverse alternative splicing events in human biology.