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Updated: Jul 7, 2026
Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food
Published on: May 6, 2013
Response of Listeria monocytogenes to liquid smoke
M Guilbaud1, I Chafsey, M-F Pilet
1UMR INRA 1014 SECALIM, ENITIAA, Nantes Cedex 3, France.
Aims:
To investigate the effect of liquid smoke on growth, survival, proteomic pattern and haemolytic potential of Listeria monocytogenes.
Methods And Results:
Growth and survival curves were recorded in brain-heart infusion broth supplemented with three concentrations of liquid smoke. L. monocytogenes growth was inhibited in the presence of 15 microg ml(-1) phenol while a rapid decrease in cell viability occurred in the presence of 30 microg ml(-1) phenol. The proteome of L. monocytogenes cytosoluble proteins was slightly modified after 2-h incubation with 30 microg ml(-1) phenol but no protein already characterized in response to other known stresses was induced, except the protease ClpP. Liquid smoke inhibited the haemolytic potential without affecting hly gene expression, showing a potential inhibition of protein activity or stability.
Conclusions:
The presence of liquid smoke in a rich medium strongly affected growth and survival of L. monocytogenes. Brief smoke stress affected the metabolic pathways and inhibited the haemolytic activity of L. monocytogenes.
Significance And Impact Of Study:
This study is a first step in the investigation of the influence of a smoked product on L. monocytogenes strains.
