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Updated: Jul 7, 2026

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
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Form, function, and fitness: pathways to survival.

James B Johnson1, D Brent Burt, Thomas J Dewitt

  • 1Department of Wildlife and Fisheries Sciences, Texas A&M University, College Station, TX 77843, USA. jamesbjohnson@tamu.edu

Evolution; International Journal of Organic Evolution
|February 13, 2008
PubMed
Summary

Anuran tadpole morphology influences survival through tail lure effects, not improved swimming. This study supports the tail lure hypothesis for predator evasion in tadpoles.

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A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
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Published on: May 21, 2020

Area of Science:

  • Evolutionary biology
  • Ecology
  • Zoology

Background:

  • Predation risk is a significant selective pressure on anuran tadpoles.
  • Two hypotheses explain how tadpole morphology may reduce predation: enhanced swimming or tail luring.

Purpose of the Study:

  • To test the hypotheses that anuran tadpole morphology reduces predation risk by improving escape swimming performance or by using the tail as a lure.
  • To investigate the direct and indirect effects of morphology on fitness, mediated by swimming performance.

Main Methods:

  • Utilized a modified morphology, performance, and fitness path analysis.
  • Included indirect effects of morphology on fitness via burst swimming speed.
  • Assessed direct paths from morphology to survival against dragonfly larvae predators.

Main Results:

  • Tadpole morphology influenced burst swimming speed, but swimming speed did not affect survival.
  • Fast tadpoles were larger, with long tails, deep tail muscles, and small bodies.
  • A tail lure morphology trait showed a direct positive relationship with survival.

Conclusions:

  • The tail lure hypothesis for predator evasion in anuran tadpoles is supported.
  • Morphology's direct effect on survival via the tail lure is significant.
  • Path analysis including direct fitness effects is crucial for evaluating selection hypotheses.