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Updated: Jun 18, 2026

Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells
Published on: June 14, 2014
X inactivation counting and choice is a stochastic process: evidence for involvement of an X-linked activator
Kim Monkhorst1, Iris Jonkers, Eveline Rentmeester
1Department of Cell Biology, Erasmus Medical Center, P.O. Box 2040, Rotterdam 3000 CA, The Netherlands; Department of Reproduction and Development, Erasmus Medical Center, P.O. Box 2040, Rotterdam 3000 CA, The Netherlands.
Abstract:
Female mammalian cells achieve dosage compensation of X-encoded genes by X chromosome inactivation (XCI). This process is thought to involve X chromosome counting and choice. To explore how this process is initiated, we analyzed XCI in tetraploid XXXX, XXXY, and XXYY embryonic stem cells and found that every X chromosome within a single nucleus has an independent probability to initiate XCI. This finding suggests a stochastic mechanism directing XCI counting and choice. The probability is directly proportional to the X chromosome:ploidy ratio, indicating the presence of an X-encoded activator of XCI, that itself is inactivated by the XCI process. Deletion of a region including Xist, Tsix, and Xite still results in XCI on the remaining wild-type X chromosome in female cells. This result supports a stochastic model in which each X chromosome in a nucleus initiates XCI independently and positions an X-encoded trans-acting XCI-activator outside the deleted region.
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