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Updated: Jul 6, 2026

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.

Asako Sakaue-Sawano¹, Hiroshi Kurokawa, Toshifumi Morimura

¹Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan.

|February 13, 2008

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Researchers developed novel fluorescent probes to visualize cell-cycle transitions, marking G1 phase red and S/G2/M phases green. This innovation allows detailed study of cell cycle dynamics in live organisms and cell cultures.

Area of Science:

Cell Biology
Genetics
Biophysics

Background:

Visualizing the G1 to S phase cell-cycle transition is challenging.
Antiphase oscillating proteins offer potential markers for cell-cycle progression.

Purpose of the Study:

To develop genetically encoded fluorescent probes for visualizing cell-cycle transitions.
To enable real-time, high-resolution imaging of cell-cycle dynamics in biological processes.

Main Methods:

Harnessing antiphase oscillating proteins to create fluorescent probes.
Developing genetically encoded probes to label G1 phase nuclei red and S/G2/M phase nuclei green.
Generating cell lines and transgenic mice constitutively expressing these probes.

Main Results:

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Last Updated: Jul 6, 2026

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

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Analysis of Multidimensional Microscopy Data Using Cell-ACDC

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Probes successfully label cell nuclei red (G1) or green (S/G2/M) in cultured cells and live mice.
Time-lapse imaging revealed spatiotemporal cell-cycle dynamics during epithelial-mesenchymal transition, neural progenitor development, and tumor growth.
Established model systems provide unprecedented spatial and temporal resolution for cell-cycle studies.

Conclusions:

The developed fluorescent probes provide a powerful tool for visualizing cell-cycle progression.
These tools facilitate the study of cell-cycle coordination with complex biological events in vivo and in vitro.
The model systems offer new avenues for understanding cell-cycle regulation in development and disease.