Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Redox compartmentalization in eukaryotic cells.

Young-Mi Go1, Dean P Jones

  • 1Emory Clinical Biomarkers Laboratory and Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Emory University, Atlanta GA 30322, USA.

Biochimica Et Biophysica Acta
|February 13, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolomic signatures of ideal cardiovascular health in black adults.

Scientific reports·2024
Same author

Protein S-palmitoylation enhances profibrotic signaling in response to cadmium.

Toxicology and applied pharmacology·2024
Same author

Pharmacometabolomics in TB Meningitis - understanding the pharmacokinetic, metabolic, and immune factors associated with anti-TB drug concentrations in cerebrospinal fluid.

medRxiv : the preprint server for health sciences·2024
Same author

Dioxin(-like)-Related Biological Effects through Integrated Chemical-wide and Metabolome-wide Analyses.

Environmental science & technology·2023
Same author

The neonatal blood spot metabolome in retinoblastoma.

EJC paediatric oncology·2023
Same author

Untargeted serum metabolomics reveals novel metabolite associations and disruptions in amino acid and lipid metabolism in Parkinson's disease.

Molecular neurodegeneration·2023
Same journal

Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Molecular Basis of Disease Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

General Subjects Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

Biochimica et biophysica acta·2019
Same journal

Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

Biochimica et biophysica acta·2019
Same journal

Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

Biochimica et biophysica acta·2019
See all related articles

Cellular compartments maintain distinct redox potentials, crucial for eukaryotic cell function. Understanding these redox control networks is key to deciphering cell processes and disease mechanisms.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Redox Biology

Background:

  • Eukaryotic cell functions rely on compartmentalization, organizing chemistry via membranes, protein complexes, and nucleic acids.
  • Cysteine residues in proteins act as key redox-sensitive elements, coordinating structural and chemical organization.
  • Central redox couples (thioredoxin-1, thioredoxin-2, glutathione/glutathione disulfide, cysteine/cystine) exist at distinct, non-equilibrium potentials across cellular compartments.

Purpose of the Study:

  • To explore the distinct redox potentials maintained in various cellular compartments (mitochondria, nuclei, secretory pathway, extracellular space).
  • To investigate the role of these compartmentalized redox potentials in cellular functions like proliferation, differentiation, and apoptosis.
  • To highlight the inseparable link between cellular redox biology and compartmentalization for understanding cell function and disease.

Related Experiment Videos

Main Methods:

  • Comparative analysis of redox potentials across different cellular compartments.
  • Examination of redox signaling pathways involving NADPH oxidases and NO synthases in the cytoplasm.
  • Correlation of changes in cytoplasmic and extracellular redox potentials with cellular functional states.

Main Results:

  • Mitochondria exhibit the most reducing environment with high electron transfer rates, while being sensitive to oxidation.
  • Nuclei possess reduced redox potentials but are more oxidation-resistant compared to mitochondria.
  • The secretory pathway utilizes oxidative systems for protein export, and extracellular compartments are maintained at stable oxidizing potentials.
  • Cytoplasmic redox potential, regulated by glutathione/glutathione disulfide, varies with proliferation, differentiation, and apoptosis.
  • Extracellular cysteine/cystine redox potential changes are linked to proliferation, cell adhesion, and apoptosis.

Conclusions:

  • Cellular redox biology is fundamentally dependent on redox compartmentalization.
  • Distinct redox potentials within cellular compartments are critical for optimizing diverse cell functions.
  • Further research into intra-compartmental redox control networks will enhance understanding of cell biology and disease pathogenesis.