Related Experiment Video
Updated: Jul 7, 2026

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
Published on: June 25, 2018
Glycosylation of S-RNases may influence pollen rejection thresholds in Solanum chacoense
Bolin Liu1, David Morse, Mario Cappadocia
1IRBV, Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, 4101 Sherbrooke est, Montréal, Québec, Canada H1X 2B2.
Abstract:
A survey of Solanum chacoense plants expressing an authentic S(11)-RNase transgene identified a line with partial compatibility to S(11) pollen. By comparing fruit set to the S-RNase levels determined immunologically in single styles, the minimum level of S(11)-RNase required for full rejection of S(11) pollen was estimated to be 18 ng per style. The S(11)-RNase threshold levels are thus considerably lower than those previously reported for the S(12)-RNase. Interestingly, these two allelic S-RNases differ dramatically in the extent of glycosylation, with the number of glycosylation sites varying from one (S(11)-RNase) to four (S(12)-RNase). It is suggested that reduced glycosylation of the S(11)-RNase may be related to the lower threshold for pollen rejection.
Related Concept Videos
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Protein Glycosylation
Glycosylation occurs in...
Proteoglycans
Responses to Salt Stress
Protein Modifications in the RER
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal sequences.

