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Basophil Activation Test for Allergy Diagnosis
Published on: May 31, 2021
Diagnostic tests based on human basophils: more potentials and perspectives than pitfalls
A L de Weck1, M L Sanz, P M Gamboa
1University of Navarra, Pamplona, Spain. alain.dew@bluewin.ch
International Archives of Allergy and Immunology
|February 13, 2008
Summary
Basophil activation tests (BAT) using flow cytometry for allergy diagnosis show more potential than pitfalls. Clinically validated studies confirm reproducible results, supporting their use in allergy diagnostics.
Area of Science:
- Immunology
- Allergy Diagnostics
Background:
- Traditional basophil activation tests (BAT) include histamine or sulfidoleukotriene release.
- Flow cytometry detection of basophil activation markers (e.g., CD63, CD203c) is a newer approach.
- Concerns exist regarding potential pitfalls of flow-cytometric BAT.
Purpose of the Study:
- To clinically validate various basophil activation tests (BAT).
- To assess the potential and pitfalls of flow-cytometric BAT.
- To review clinically validated studies on BAT for diverse allergens and conditions.
Main Methods:
- Clinical validation of various BAT over several years.
- Comprehensive review of clinically validated studies.
- Analysis of studies covering aeroallergens, insect venoms, latex, food allergens, and drugs (myorelaxants, beta-lactams, pyrazolones, NSAIDs), and chronic urticaria.
Main Results:
- Flow-cytometric BAT demonstrate more potential and perspectives than pitfalls.
- Reproducible and meaningful results are obtainable with various BAT protocols.
- Studies show successful application across a wide range of allergens and clinical conditions.
Conclusions:
- Clinically indicated BAT, including flow-cytometric methods, can be reliably performed.
- Despite the need for optimization and standardization, there are no reasons to withhold BAT from allergic patients.
- Laboratories with allergy and flow cytometry expertise can effectively utilize these diagnostic tools.

