[Catechol biosensor based on immobilizing laccase to modified core-shell magnetic nanoparticles supported on carbon paste electrode]
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Updated: Jan 31, 2026

Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection
Published on: May 13, 2019
Yi Zhang1, Guang-ming Zeng, Lin Tang
1College of Environmental Science and Engineering, Hunan University, Changsha 410082, China. ezhangyi123@yahoo.com.cn
Abstract:
A catechol biosensor was developed and used to analyze compost extracts based on the immobilization of laccase on the surface of modified magnetic core-shall (Fe3O4-SiO2) nanoparticles. Laccase was convalently immobilized on the magnetic nanoparticles by glutaraldehyde, which were modified with amino groups on its surface. The resulting magnetic bio-nanoparticles were attached to the surface of carbon paste electrode with the help of a permanent magnet to determine catechol. The immobilization matrix provided a good microenvironment for retaining the bioactivity of laccase. The linear range for catechol determination was 7.5 x 10(-7)-2.75 x 10(-4) mol/L, with a detection limit of 7.5 x 10(-7) mol/L. The detection current reached 95% of the steady-state current within about 70 s. Catechol concentration in compost extracts were determined by laccase biosensor and HPLC, with approximately the same result.
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