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A comparison between a time domain and continuous wave small animal optical imaging system
S Keren1, O Gheysens, C S Levin
1Radiology Department, Stanford University, Standford, CA 94305, USA. shayk@stanford.edu
IEEE Transactions on Medical Imaging
|February 14, 2008
Summary
The eXplore Optix time-domain system offers superior sensitivity and depth for small animal fluorescence imaging compared to the IVIS 200. However, it requires longer acquisition times and cannot image multiple subjects simultaneously.
Area of Science:
- Biomedical Imaging
- Optical Imaging
- Small Animal Imaging
Background:
- Small animal imaging is crucial for preclinical research.
- Existing continuous wave planar imaging systems have limitations in sensitivity and depth.
- Time-domain tomography offers potential advancements in fluorescence imaging.
Purpose of the Study:
- Evaluate the performance of the eXplore Optix, a novel time-domain tomography system.
- Compare its capabilities against the widely used IVIS 200 continuous wave planar system.
- Assess sensitivity, detection depth, and spatial resolution for small animal fluorescence imaging.
Main Methods:
- Phantom study utilizing eXplore Optix and IVIS 200 systems.
- Point-wise illumination and collection scheme for eXplore Optix.
- Wide-area illumination for IVIS 200.
- Time-resolved detection of fluorescence photons.
Main Results:
- eXplore Optix demonstrated a log order higher sensitivity and significantly greater detection depth.
- Superior spatial resolution was observed with the eXplore Optix.
- Time-resolved detection enabled fluorescence lifetime, absorption mapping, and depth estimation.
Conclusions:
- The eXplore Optix system significantly outperforms the IVIS 200 in sensitivity, depth, and resolution for small animal fluorescence imaging.
- Key advantages include quantitative volumetric distribution of fluorophore concentration.
- Increased acquisition time and single-animal imaging are current limitations of the eXplore Optix.
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