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Nuclease tolerant FRET probe based on DNA-quantum dot conjugation

Daisuke Onoshima1, Noritada Kaji, Manabu Tokeshi

  • 1Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya, Japan. onoshima.daisuke@d.mbox.nagoya-u.ac.jp

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|February 14, 2008
PubMed

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...