Related Experiment Video
Updated: Jul 7, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Microscopic measurement of circular dichroism spectra
Aira Matsugaki1, Hideaki Takechi, Hideaki Monjushiro
1Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan.
Abstract:
A microscope device to measure the circular dichroism (CD) spectra of a specified microscopic region of chiral samples was constructed by combining of a couple of objective lenses and a CCD camera, which was installed in a sample chamber of a commercially available CD spectropolarimeter. By using this apparatus, high quality micro-CD spectra in the 60 x 60 microm region of samples could be measured. Micro-CD spectra of thin film of chiral DNA samples on glass and a natural kidney bean leaf were measured, and the potential of the micro-CD apparatus was successfully demonstrated.
More Related Videos
08:54Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy
Published on: June 5, 2019
14:09Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
Published on: April 7, 2014
Related Concept Videos
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Spectrophotometry: Introduction
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Measuring Reaction Rates
UV–Vis Spectrometers
UV–Vis Spectroscopy: Molecular Electronic Transitions
UV–Vis Spectroscopy: Woodward–Fieser Rules