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Updated: Jul 7, 2026
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Towards multidimensional liquid chromatography separation of proteins using fluorescence and isotope-coded protein
Florian Tribl1, Christiane Lohaus, Tanja Dombert
1Medizinisches Proteom-Center, Ruhr-Universität Bochum, Bochum, Germany.
Abstract:
HPLC has emerged as a valuable tool for separating proteins. To address the analysis of complex proteomes and quantitative changes of proteins therein, we developed a multidimensional LC (MDLC)-based approach followed by large gel 1-D SDS-PAGE. Here we present a novel strategy that allows for simultaneously identifying and quantifying differentially regulated proteins following three separation and fractionation steps. This MDLC platform integrates advantages of dual protein labelling using both fluorescence and isotope-coded tags for subsequent detection and quantification of abundance ratios of proteins by MS.
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