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Updated: Jul 7, 2026
Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
Published on: July 17, 2021
[Expression of recombinant bovine beta-interferon by baculovirus system and evaluation of its bioactivity]
Wei-Ye Chen1, Hui-Jun Zhao, Xi-Jun Wang
1College of Veterinary Medicine, Nanjing agricultural University, Nanjing 210095, China. chenweiye1980@yahoo.com.cn
Abstract:
In this paper, a recombinant baculovirus containing the ORF of bovine interferon-beta (BoIFN-beta) gene, rBac-BoIFN-beta, was generated to express recombinant BoIFN-beta (rBoIFN-beta) in sf9 insect cells. The expression of rBoIFN-beta in rBac-BoIFN-beta infecting sf9 cells and its supernatants was confirmed by indirect immunofluorescence assay and Western blot. The antiviral activity of rBoIFN-beta in the supernatant can reach 10(6.0) AU/mL evaluated by the antiviral assay with VSV * GFP that expressed green fluorescence protein, and rBoIFN-beta could stimulate the expression of luciferase reporter gene controlled by chicken Mx promoter. All the results showed that rBac-BoIFN-beta constructed here could express high level recombinant BoIFN-beta in secreted form that had the bioactivity of natural type I IFN.
