Related Experiment Video
Updated: Jul 7, 2026

Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane (SSM)-Based Electrophysiology
Published on: May 3, 2021
[Sodium ion transportation system and its possible mechanisms in bacteria]
Li-Fu Yang1, Bai-Suo Zhao, Su-Sheng Yang
1Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Physiology for Tropical Crops of Ministry of Agriculture, Danzhou 571737, China. ylfri@126.com
Abstract:
Sodium ion with high concentration is toxic to living cells, and microorganisms adapt to the environment containing high concentration of salt by the strategies of salt-in-cytoplasm and compatible solutes. The Na+ extrusion system plays important roles in maintaining cytoplasmic Na+ homeostasis and pH level in microbial cells. Two possible mechanisms of Na+ circulation across the cytoplasmic membrane have been proposed, namely primary Na+ pump and secondary Na+/H+ antiporter. Primary sodium pumps coupled the extrusion of Na+ to respiration, and the activity of which was insensitive to uncoupler CCCP ( carbonyl-cyanide m-chlorophenylhydrazone). There were two types of secondary Na+/H+ antiporters-encoding genes designated single gene and multiple subunits, respectively. The types of transportation systems for Na+, possible mechanisms of Na+ extrusion, and projects for further study in bacteria are reviewed.
Related Concept Videos
Gram-negative Bacterial Protein Secretion Systems
Bacterial Translocation and Protein Secretion
Electron Transport Chain Components
Ion Channels
Ion channels are specialized integral membrane proteins on the plasma membrane that allow specific...
Secondary Active Transport
Secondary Active Transport

