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Updated: Jul 7, 2026

T4 Bacteriophage and E. coli Interaction in the Murine Intestine: A Prototypical Model for Studying Host-Bacteriophage Dynamics In Vivo
Published on: January 26, 2024
Rapid isolation and identification of bacteriophage T4-encoded modifications of Escherichia coli RNA polymerase: a
Lars F Westblade1, Leonid Minakhin, Konstantin Kuznedelov
1The Rockefeller University, New York, New York 10065, USA.
Abstract:
Bacteriophages are bacterial viruses that infect bacterial cells, and they have developed ingenious mechanisms to modify the bacterial RNA polymerase. Using a rapid, specific, single-step affinity isolation procedure to purify Escherichia coli RNA polymerase from bacteriophage T4-infected cells, we have identified bacteriophage T4-dependent modifications of the host RNA polymerase. We suggest that this methodology is broadly applicable for the identification of bacteriophage-dependent alterations of the host synthesis machinery.
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