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Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
Published on: March 11, 2020
A rapid and efficient inoculation method for Tomato spotted wilt tospovirus
B Mandal1, A S Csinos, N Martinez-Ochoa
1Department of Plant Pathology, University of Georgia, Coastal Plain Experiment Station, Tifton, GA 31793, USA.
Journal of Virological Methods
|February 15, 2008
Summary
A novel spray inoculation method rapidly infects plants with Tomato spotted wilt virus (TSWV). This efficient technique significantly speeds up TSWV inoculation for faster resistance screening.
Area of Science:
- Plant Pathology
- Virology
- Agricultural Science
Background:
- Tomato spotted wilt virus (TSWV) causes significant crop losses globally.
- Accurate and efficient inoculation methods are crucial for studying TSWV and developing resistant varieties.
- Traditional hand-inoculation methods are time-consuming and labor-intensive.
Purpose of the Study:
- To develop a rapid and efficient spray inoculation method for TSWV.
- To evaluate the effectiveness of this method on different host plants.
- To compare the speed of spray inoculation with traditional hand inoculation.
Main Methods:
- A spray device combining a spray gun, atomizer, and CO2-powered sprayer was used.
- Inoculum consisted of infected leaf sap with additives (phosphate buffer, sodium sulfite, 2-mercaptoethanol, Celite, Carborundum).
- Chilled inoculum was applied at 1.1 ml/plant with 4.1 bar air pressure.
Main Results:
- Nearly 100% systemic infection was achieved in tobacco (Nicotiana tabacum) plants.
- High infection rates (75.0-100% and 72.2-91.6%) were observed in peanut (Arachis hypogaea) and tomato (Lycopersicon esculentum), respectively.
- The spray method was estimated to be approximately 50 times faster than hand inoculation.
Conclusions:
- The developed spray inoculation method is rapid, efficient, and effective for TSWV.
- This technique allows for simultaneous inoculation of numerous plants, facilitating large-scale germplasm and breeding line screening.
- The method is suitable for diverse TSWV host species, aiding in resistance breeding programs.
