Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bacteriophage lambda P gene shows host killing which is not dependent on lambda DNA replication.

S Maiti1, M Mukhopadhyay, N C Mandal

  • 1Department of Biochemistry, Bose Institute, Calcutta, India.

Virology
|May 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Endovascular Aneurysm Repair for Infrarenal Abdominal Aortic Aneurysm: New Horizon in Endovascular Surgery in Bangladesh.

Mymensingh medical journal : MMJ·2024
Same author

Highly Ordered Monolayers of μm-Sized Polystyrene Spheres Studied by Grazing-Incidence Small-Angle X-ray Scattering, Simulations, and Geometrical Calculations.

Langmuir : the ACS journal of surfaces and colloids·2024
Same author

Screening of osmotic stress-tolerant bacteria for plant growth promotion in wheat (Triticum aestivum L.) and brinjal (Solanum melongena L.) under drought conditions.

Letters in applied microbiology·2022
Same author

Training programme in gasless laparoscopy for rural surgeons of India (TARGET study) - Observational feasibility study.

International journal of surgery open·2021
Same author

Spatio-temporal evolution of magnetohydrodynamic blood flow and heat dynamics through a porous medium in a wavy-walled artery.

Computers in biology and medicine·2021
Same author

Effect of macro-calcification on the failure mechanics of intracranial aneurysmal wall tissue.

Experimental mechanics·2021
Same journal

Unveiling the hidden virome of Sclerotinia sclerotiorum: New tools and discoveries in mycovirus detection.

Virology·2026
Same journal

ASFV pDP238L negatively regulates type I interferon production via inhibiting the methylation of TBK1.

Virology·2026
Same journal

Divergent poxvirus identified in a non-native black rat from Madagascar.

Virology·2026
Same journal

Genomic and functional characterization of Bacillus phage BCE1 targeting a key gut bacterium in Aedes albopictus larvae.

Virology·2026
Same journal

Viral etiology of orogenital papillomatosis and squamous cell carcinoma in bottlenose dolphins in the southeastern United States.

Virology·2026
Same journal

Orthoflaviviruses in the modern era: Challenges and breakthroughs.

Virology·2026
See all related articles

A specific mutation in bacteriophage lambda

Area of Science:

  • Molecular Biology
  • Virology
  • Genetics

Background:

  • Bacteriophage lambda's cro gene regulates gene expression.
  • Host cell killing by bacteriophages is a complex process.
  • The N protein is crucial for late gene expression in lambda phage.

Purpose of the Study:

  • To investigate the mechanism of host cell killing by a specific bacteriophage lambda mutation.
  • To determine the role of the cro gene and P protein in phage-induced lethality.
  • To elucidate how mutations in cro affect gene regulation and host interactions.

Main Methods:

  • Site-directed mutagenesis to create the hk mutation in the cro gene.
  • Cloning of lambda DNA fragments containing cro and other genes into plasmid vectors.

Related Experiment Videos

  • Analysis of gene expression and protein activity in infected bacterial hosts.
  • Construction and study of plasmid derivatives with specific gene deletions.

    • Main Results:

    • The hk mutation in cro leads to host killing under N- conditions.
    • The hk mutation causes reduced Cro activity and increased P gene expression.
    • Host killing is dependent on the presence of a functional P gene and elevated P protein levels.
    • Deletion of the tR1 terminator also results in host killing due to elevated P expression.
    • The hk mutation impairs Cro's negative regulation of the pR promoter.

    Conclusions:

    • Elevated P protein levels, caused by the hk mutation or tR1 deletion, are lethal to the host.
    • The hk mutation renders Cro protein partially defective in repressing pR, leading to lethal P expression.
    • P protein-induced host killing is independent of phage DNA replication and other phage gene functions.