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Updated: Jul 7, 2026

Aseptic Laboratory Techniques: Plating Methods

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Published on: May 11, 2012

Evaluation of an Automated Instrument for Inoculating and Spreading Samples onto Agar Plates.

J H Glasson¹, L H Guthrie, D J Nielsen

¹Institute of Medical and Veterinary Science, Frome Road, Adelaide, South Australia. john.glasson@imvs.sa.gov.au

Journal of Clinical Microbiology

|February 15, 2008

Summary

A new automated instrument for microbiological analysis significantly improves agar plate inoculation and spreading. This method yields up to 44% more colonies and enhances detection limits for clinical specimens.

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The Soft Agar Colony Formation Assay

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Area of Science:

Microbiology
Medical Technology
Laboratory Automation

Background:

Manual inoculation and spreading of microbiological specimens onto agar plates can be labor-intensive and may lead to inconsistent sample distribution.
Current methods may not optimize the use of agar surface area, potentially reducing colony yield and limiting detection sensitivity.

Purpose of the Study:

To evaluate a novel automated instrument for the inoculation and spreading of microbiological specimens onto agar plates.
To compare the efficiency and effectiveness of the automated instrument against the traditional manual loop-to-plate method.

Main Methods:

The instrument automatically selects agar plates (full or biplates) from input cassettes.
Specimens are inoculated and spread using a novel plastic applicator, followed by automated plate labeling and sorting.
Comparison with manual loop-to-plate inoculation was performed using clinical specimens and standard cultures.

Main Results:

The instrument inoculated and spread samples over a larger agar surface area compared to manual methods.
Up to 44% more usable colonies were obtained per plate using the automated instrument.
Viable counts demonstrated detection of as few as 10(2) CFU/ml in fluids, with improved enumeration of organisms in urine specimens.

Conclusions:

The automated instrument offers a more efficient and effective method for microbiological specimen inoculation and spreading.
Increased colony yield and enhanced detection sensitivity suggest significant advantages for clinical diagnostics and research.
This technology has the potential to improve laboratory workflow and diagnostic accuracy in microbiology.