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Updated: Jul 7, 2026

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Published on: June 30, 2022
Activation of protein splicing with light in yeast
Amy B Tyszkiewicz1, Tom W Muir
1Laboratory of Synthetic Protein Chemistry, The Rockefeller University, 1230 York Ave., New York, New York 10065, USA.
Abstract:
Spatiotemporal regulation of protein function is a key feature of living systems; experimental tools that provide such control are of great utility. Here we report a genetically encoded system for controlling a post-translational process, protein splicing, with light. Studies in Saccharomyces cerevisiae demonstrate that fusion of a photodimerization system from Arabidopsis thaliana to an artificially split intein permits rapid activation of protein splicing to yield a new protein product.
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