Related Experiment Video
Updated: Jul 7, 2026
Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
Published on: January 20, 2015
Changes of 5-hydroxytryptamine and tryptophan hydroxylase expression in the ventral horn of spinal cord
Chuan-Xiang Xu1, Hong-Tao Liu, Jing Wang
1Institute of Healthy and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
Objective:
To investigate changes of 5-hydroxytryptamine (5-HT) and its synthesis rate-limiting enzyme tryptophan hydroxylase (TPH) in the ventral horn of spinal cord after exercise-induced fatigue, and to further discuss the mechanism of exercise-induced central fatigue at spinal level.
Methods:
Sixteen healthy adult Wistar rats were randomly divided into 2 groups: exercise-induced fatigue group and control group. Immunohistochemical staining for 5-HT and TPH in the ventral horn were performed and analyzed quantitatively. The mean optic densities of 5-HT and TPH positive fibers or terminals were measured by computerized image analyzer.
Results:
Both 5-HT and TPH positive fibers/terminals decreased in the exercise-induced fatigue group. The immunohistochemical staining was weaker and the mean optic densities decreased obviously in the fatigue group compared with those in the control group (P< 0.05).
Conclusion:
5-HT and TPH in the ventral horn of spinal cord might be involved in exercise-induced fatigue.
