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Related Experiment Video

Updated: Jul 7, 2026

Site-Specific Lysine Lactylation via Genetic Code Expansion in E. coli and Mammalian Cells

Site-Specific Lysine Lactylation via Genetic Code Expansion in E. coli and Mammalian Cells

Published on: February 24, 2026

Lactate modulates gene expression in human mesenchymal stem cells.

Derek Zieker¹, Richard Schäfer, Jörg Glatzle

¹Department of General and Transplant Surgery, University of Tuebingen, Tuebingen, Germany. derek.zieker@med.uni-tuebingen.de

Langenbeck'S Archives of Surgery

|February 15, 2008

Summary

This summary is machine-generated.

Lactate influences gene expression in mesenchymal stem cells (MSCs), impacting wound healing pathways. This study reveals lactate

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Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Published on: March 8, 2019

Area of Science:

Biochemistry
Cell Biology
Regenerative Medicine

Background:

Surgical wounds exhibit elevated lactate levels.
Lactate promotes collagen synthesis, angiogenesis, and stem cell homing.
The effect of lactate on mesenchymal stem cell (MSC) gene expression is not well understood.

Purpose of the Study:

To investigate the impact of lactate on the gene expression of human bone marrow-derived MSCs.
To test the hypothesis that lactate influences MSC gene expression profiles.

Main Methods:

MSCs were isolated from human bone marrow.
MSCs were treated with 15 mM lactate for various durations (1h to 7 days).
Gene expression analysis was performed using microarrays; MSC phenotype was assessed via FACS.

Main Results:

Lactate significantly altered the expression of numerous genes, including those related to cytokines, transcription factors, and cell-matrix interactions.
Upregulation of interleukin-6, heat shock protein 70, and hypoxia-inducible factor-1alpha was observed.
Downregulation of superoxide dismutase 2 and BCL2-associated X protein was noted; MSC surface markers remained unchanged.

Conclusions:

Lactate modulates gene expression in MSCs, particularly affecting pathways involved in wound healing.
Lactate treatment did not significantly alter the overall phenotype of MSCs.
Findings offer insights into wound healing and suggest potential therapeutic strategies for non-healing wounds.