Related Experiment Video
Updated: Jul 7, 2026
Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis
Published on: February 5, 2014
Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight
I G Hamoy1, E J M Santos, S E B Santos
1Laboratório de Genética Humana e Médica, Centro de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil. ighamoy@yahoo.com.br
Abstract:
The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtained demonstrate high heterozygosity (average of 0.69) in a Lower Amazon population. The multiplex system described can thus be considered a fast, efficient and inexpensive method for the investigation of genetic variability in Arapaima populations.
More Related Videos
10:17Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
10:33Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
Published on: June 17, 2019