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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jul 7, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

miChip: an array-based method for microRNA expression profiling using locked nucleic acid capture probes.

Mirco Castoldi1, Sabine Schmidt, Vladimir Benes

  • 1Molecular Medicine Partnership Unit (MMPU), Im Neuenheimer Feld 153, Heidelberg 69120, Germany.

Nature Protocols
|February 16, 2008
PubMed
Summary
This summary is machine-generated.

This study introduces miChip, a microarray platform using locked nucleic acid probes for accurate and sensitive genome-wide microRNA (miRNA) profiling. The 3-day protocol overcomes challenges in detecting short, low-copy number miRNAs.

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Last Updated: Jul 7, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Performing Custom MicroRNA Microarray Experiments
07:04

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • MicroRNAs (miRNAs) are short noncoding RNAs regulating gene expression post-transcriptionally.
  • Microarray analysis of miRNAs faces challenges due to short sequence length, low copy numbers, and variable melting temperatures.
  • Existing methods struggle with accurate and sensitive miRNA detection.

Purpose of the Study:

  • To present a detailed protocol for the miChip microarray platform for genome-wide miRNA profiling.
  • To demonstrate the application of locked nucleic acid (LNA)-modified capture probes for enhanced miRNA detection.
  • To provide a comprehensive workflow from tissue sample to miRNA expression data.

Main Methods:

  • Development of the miChip microarray platform utilizing LNA-modified capture probes.
  • Preparation and fluorescent labeling of small RNA from total RNA.
  • Hybridization of labeled small RNA to immobilized LNA probes and post-hybridization processing.
  • Protocol duration from intact tissue to data generation is approximately 3 days.

Main Results:

  • The miChip platform enables highly accurate and sensitive genome-wide miRNA expression profiling.
  • LNA-modified probes significantly improve detection of short and low-abundance miRNAs.
  • The protocol provides reliable data within a 3-day timeframe.

Conclusions:

  • The miChip platform offers a robust solution for overcoming technical challenges in miRNA microarray analysis.
  • This method facilitates precise quantification of miRNA expression levels across the genome.
  • The developed protocol is efficient and suitable for generating high-quality miRNA expression data from biological samples.