Ribosomal proteins are targets for the NEDD8 pathway
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Updated: Jul 7, 2026

Detection of Protein Ubiquitination
Published on: August 19, 2009
Dimitris P Xirodimas1, Anders Sundqvist, Akihiro Nakamura
1Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK. d.xirodimas@dundee.ac.uk
Abstract:
Identification of the molecular targets for post-translational modifications is an important step for explaining the regulated pathways. The ubiquitin-like molecule NEDD8 is implicated in the regulation of cell proliferation, viability and development. By combining proteomics and in vivo NEDDylation assays, we identified a subset of ribosomal proteins as novel targets for the NEDD8 pathway. We further show that the lack of NEDDylation in cells causes ribosomal protein instability. Our studies identify a novel and specific role of the NEDD8 pathway in protecting a subset of ribosomal proteins from destabilization.
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