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Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes
Published on: December 19, 2020
A highly efficient construction of GM1 epitope tetrasaccharide and its conjugation with KLH
Takeru Yoshikawa1, Yuki Kato, Nobuhiro Yuki
1Department of Applied Bio-organic Chemistry, Gifu University, Gifu 501-1193, Japan.
Glycoconjugate Journal
|February 16, 2008
Abstract:
GM1 epitope tetrasaccharide was synthesized by a condensation of sialyl-alpha(2-3)-gal acceptor and gal-beta(1-3)-GalN donor in a highly efficient manner. After introduction of mercaptohexanol to the tetrasaccharide, it was coupled to maleimide-activated KLH carrier protein to give the desired GM1 epitope-KLH conjugate.
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