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Related Concept Videos

Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance01:25

Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance

In healthy individuals, serum creatinine levels remain stable due to a balance between its constant production—primarily from muscle metabolism—and renal excretion. Creatinine is freely filtered by the glomeruli, making it a valuable marker for estimating renal function. When the glomerular filtration rate (GFR) decreases, the kidneys can only eliminate less creatinine, causing serum levels to rise.Serum creatinine concentration is widely used to estimate creatinine clearance (Clcr), a...
Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration01:28

Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration

Glomerular filtration rate (GFR) can be estimated from serum creatinine using the modification of diet in renal disease (MDRD) formula or the chronic kidney disease–epidemiology collaboration (CKD–EPI) equation. Both methods are widely used in clinical practice to assess kidney function and guide treatment decisions.The MDRD equation does not require weight or height measurements and is normalized to the body surface area of 1.73 m², considered the average adult surface area. This equation is...
Renal Drug Clearance: Comparison Between Renal Excretion Methods01:08

Renal Drug Clearance: Comparison Between Renal Excretion Methods

Renal clearance is a critical parameter encompassing kidney filtration, secretion, and reabsorption processes. It is calculated using a specific equation to determine the rate at which the kidneys clear a drug.
Renal clearance is often associated with the renal glomerular filtration rate (GFR), which represents the rate at which plasma is filtered through the glomeruli in the kidney. When drug reabsorption is minimal and there is no active secretion, renal clearance is closely related to the...
Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate01:25

Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate

The glomerular filtration rate (GFR) is a critical indicator of kidney health, reflecting how well the kidneys filter blood. Changes in GFR can signal potential kidney impairment, necessitating accurate measurement methods to monitor kidney function effectively.Various molecules can serve as markers for GFR measurement, with the ideal marker meeting several specific criteria. It must freely filter at the glomerulus, avoid reabsorption or secretion by the renal tubules, remain unmetabolized, not...
Factors Affecting Renal Clearance: Renal Impairment01:17

Factors Affecting Renal Clearance: Renal Impairment

Renal dysfunction significantly impairs the renal clearance of drugs, leading to potential complications in drug therapy. Renal failure, which can be caused by various factors, poses a significant challenge in the elimination of drugs from the body.
One condition associated with renal failure is uremia. Uremia is characterized by impaired glomerular filtration and fluid accumulation in the body. This condition hinders the renal clearance of drugs, resulting in drug accumulation and potential...
Determination of Renal Drug Clearance: Graphical and Midpoint Methods01:07

Determination of Renal Drug Clearance: Graphical and Midpoint Methods

Renal clearance, a crucial parameter in pharmacokinetics, can be determined using two different methods: the graphical method and the midpoint method. These methods provide insights into the rate of drug excretion by the kidneys and aid in assessing renal function.
The graphical method involves plotting the rate of drug excretion in urine against the plasma drug concentration. By analyzing the graph, the clearance can be calculated and obtained. Drugs rapidly excreted by the kidneys exhibit a...

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Related Experiment Video

Updated: Jul 7, 2026

Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice
09:09

Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice

Published on: August 9, 2013

Delay in separating blood samples affects creatinine measurement using the Roche kinetic Jaffe method.

Loretta Ford1, Jonathan Berg

  • 1Clinical Biochemistry Department, City Hospital, Dudley Road, Birmingham, UK. Loretta.Ford@swbh.nhs.uk

Annals of Clinical Biochemistry
|February 16, 2008
PubMed
Summary

Delayed serum separation significantly increases creatinine levels, impacting kidney disease staging. Avoid reporting creatinine results after sample separation delays to prevent misclassification.

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A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
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A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

Published on: May 10, 2013

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Last Updated: Jul 7, 2026

Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice
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Published on: August 9, 2013

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
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A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

Published on: May 10, 2013

Area of Science:

  • Clinical Chemistry
  • Nephrology
  • Laboratory Medicine

Background:

  • Serum creatinine measurement is crucial for assessing kidney function.
  • The kinetic Jaffe method is commonly used for creatinine analysis.
  • Accurate estimated glomerular filtration rate (eGFR) is vital for chronic kidney disease (CKD) staging.

Purpose of the Study:

  • To investigate the impact of delayed sample separation on serum creatinine levels.
  • To evaluate the effect of delayed separation on eGFR calculations.
  • To determine the consequences for CKD staging.

Main Methods:

  • Analyzed clotted blood samples with delayed separation (up to 48 hours) using the Roche kinetic Jaffe method.
  • Compared creatinine measurements from samples separated at baseline (30 min) versus after 24 hours.
  • Calculated eGFR using the simplified Modification of Diet in Renal Disease formula for CKD staging.

Main Results:

  • Delayed separation (16-48 hours) led to significant creatinine increases (mean 29% by 48h).
  • A 24-hour delay in outpatient samples showed a mean creatinine increase of 11% with wide individual variation.
  • CKD staging was altered for 32% of patients, with shifts from stage 1 to 2 and stage 2 to 3.

Conclusions:

  • Delayed sample separation significantly affects serum creatinine measurements with the kinetic Jaffe method.
  • This delay can lead to misclassification of CKD stages.
  • Laboratories should refrain from reporting creatinine results with delayed separation and update practice guidelines accordingly.