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Direct binding of protein A, protein G, and anti-IgG conjugates to chicken infectious anemia virus
1Department of Avian and Aquatic Animal Medicine, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Recombinant Protein A, recombinant Protein G, and anti-chicken-IgG anti-bodies raised in rabbits, goats, or horses were found to bind directly to chicken infectious anemia virus (CIAV). MSB-1 cells infected with the Cux-1 strain of chicken anemia agent, but not to uninfected MSB-1 cells were found to react with fluorescein isothiocyanate conjugates. In an enzyme-linked immunosorbent assay, rabbit anti-chicken horseradish peroxidase conjugate bound directly to CIA-1 CIAV-coated plates. In addition, sera from a low percentage of specific-pathogen-free breeder hens reacted in an indirect fluorescent antibody test to detect CIAV antibodies. These reactions generally disappeared within a month. The breeder flocks were demonstrated to be free of CIAV infection by the susceptibility of their progeny.
Recombinant Protein A, recombinant Protein G, and anti-chicken-IgG anti-bodies raised in rabbits, goats, or horses were found to bind directly to chicken infectious anemia virus (CIAV). MSB-1 cells infected with the Cux-1 strain of chicken anemia agent, but not to uninfected MSB-1 cells were found to react with fluorescein isothiocyanate conjugates. In an enzyme-linked immunosorbent assay, rabbit anti-chicken horseradish peroxidase conjugate bound directly to CIA-1 CIAV-coated plates. In addition, sera from a low percentage of specific-pathogen-free breeder hens reacted in an indirect fluorescent antibody test to detect CIAV antibodies. These reactions generally disappeared within a month. The breeder flocks were demonstrated to be free of CIAV infection by the susceptibility of their progeny.