Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Translesion DNA Polymerases02:10

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Base Excision Repair01:54

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
Base Excision Repair01:54

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multifaceted roles of PDS5B in RAD51-dependent homology-directed DNA repair and replication fork protection.

Nature communications·2026
Same author

Structural insight into how RAD51 paralog exchange regulates RAD51 filament formation.

Nature structural & molecular biology·2026
Same author

Resolution of R-loops and transcription-replication conflicts by SETX-BRCA1-BARD1 complex.

Nature structural & molecular biology·2026
Same author

Cryo-EM structures of UBA6 reveal mechanisms of E1-E2 specificity and dual FAT10/ubiquitin thioester transfer.

Nature communications·2026
Same author

High-throughput mapping of 6,888 <i>RAD51D</i> variants identifies distinct biochemical functions needed for homologous recombination and olaparib response.

bioRxiv : the preprint server for biology·2026
Same author

Phosphoregulation of RAD51AP1 function in homology-directed repair.

The Journal of biological chemistry·2026

Related Experiment Video

Updated: Jul 7, 2026

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
10:59

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

Structural insights into DNA lesion bypass.

Patrick Sung

    Structure (London, England : 1993)
    |February 16, 2008
    PubMed
    Summary
    This summary is machine-generated.

    Specialized DNA polymerases can replicate through DNA lesions. Yeast Rev1, a key DNA repair polymerase, is shown to bypass DNA damage effectively.

    More Related Videos

    Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
    07:55

    Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

    Published on: September 11, 2022

    Related Experiment Videos

    Last Updated: Jul 7, 2026

    Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
    10:59

    Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

    Published on: May 24, 2017

    Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
    07:55

    Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

    Published on: September 11, 2022

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Structural Biology

    Background:

    • DNA damage poses a significant threat to genomic integrity, necessitating specialized cellular mechanisms for repair and replication.
    • DNA polymerases are crucial enzymes for DNA replication and repair, with some possessing unique abilities to navigate damaged DNA segments.

    Discussion:

    • The study investigates the structural and mechanistic basis of DNA lesion bypass by yeast Rev1, a specialized DNA polymerase.
    • Nair et al. elucidate how Rev1 interacts with and replicates across DNA lesions, preventing replication fork stalling.

    Key Insights:

    • Structural analysis reveals the mechanism by which yeast Rev1 achieves DNA lesion bypass.
    • The findings provide atomic-level insights into the function of a critical enzyme in DNA damage tolerance pathways.

    Outlook:

    • Understanding Rev1's mechanism can inform the development of novel therapeutic strategies targeting DNA repair pathways in diseases like cancer.
    • Further research into other lesion-replicating polymerases may uncover broader principles of DNA damage tolerance and repair.