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Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein
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A perchlorate sensitive iodide transporter in frogs.

Deborah L Carr1, James A Carr, Ray E Willis

  • 1Department of Cell Physiology and Molecular Biophysics, Texas Tech University Health Sciences Center, 3601 4th Street, Lubbock, TX 79430, USA.

General and Comparative Endocrinology
|February 16, 2008
PubMed
Summary

Researchers identified a novel sodium-iodide symporter in African clawed frogs (Xenopus laevis). This gene product functions as an iodide transporter, crucial for thyroid hormone synthesis.

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Area of Science:

  • Molecular Biology
  • Comparative Physiology
  • Biochemistry

Background:

  • Solute carrier family proteins are vital membrane transporters.
  • Identifying novel transporters aids understanding of physiological processes.
  • Amphibian genomes offer insights into conserved biological functions.

Purpose of the Study:

  • To identify and characterize a putative iodide transporter in Xenopus laevis.
  • To confirm the functional role of the identified gene product in iodide uptake.
  • To investigate the phylogenetic relationship and tissue distribution of the transporter.

Main Methods:

  • Heterologous expression of Xenopus laevis nucleotide sequence in mammalian cells.
  • Measurement of radioactive iodide (125I) uptake.
  • Inhibition assays using perchlorate.
  • Analysis of tissue-specific mRNA expression.
  • Phylogenetic analysis.

Main Results:

  • Transfected mammalian cells exhibited significantly increased 125I uptake compared to controls.
  • Iodide uptake was inhibited by perchlorate, indicating a Na+/iodide symporter mechanism.
  • mRNA expression was detected in thyroid, stomach, kidney, and ovaries of Xenopus laevis and Rana catesbeiana.
  • Phylogenetic analysis confirmed orthology with vertebrate Na+-dependent iodide symporters.

Conclusions:

  • The identified Xenopus laevis sequence encodes a functional sodium-iodide symporter.
  • This transporter plays a role in iodide uptake in amphibians, analogous to vertebrate counterparts.
  • The findings expand our understanding of iodide transport mechanisms across species.