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Ibuprofen metabolite profiling using a combination of SPE/column-trapping and HPLC-micro-coil NMR
Danijel Djukovic1, Emmanuel Appiah-Amponsah, Narasimhamurthy Shanaiah
1Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Abstract:
Solid-phase extraction and column-trapping preconcentration are combined to enhance HPLC-nuclear magnetic resonance (HPLC-NMR) and applied to metabolite profiling in biological samples. Combining the two signal enhancement techniques improved the NMR signal substantially such that we were able to identify 2-hydroxyibuprofen, carboxyibuprofen, and unmetabolized ibuprofen molecules from a small urine sample after a therapeutic dose of ibuprofen. The hyphenated SPE/column-trapping method resulted in an excellent overall signal enhancement of up to 90-fold.
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