Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 7, 2026

Subconjunctival Administration of Adeno-associated Virus Vectors in Small Animal Models

Subconjunctival Administration of Adeno-associated Virus Vectors in Small Animal Models

Published on: March 16, 2022

Novel AAV serotypes for improved ocular gene transfer.

Corinna Lebherz¹, Albert Maguire, Waixing Tang

¹Gene Therapy Program, Division of Medical Genetics, Department of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

The Journal of Gene Medicine

|February 19, 2008

Summary

Novel adeno-associated virus (AAV) serotypes 7 and 8 demonstrate superior gene transfer efficiency and long-term expression in ocular tissues compared to existing AAV vectors. These findings advance gene therapy for inherited and acquired ocular diseases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

High-sensitivity troponin T as a rule-out marker for myocardial inflammation detectable by CMR imaging.

Open heart·2025

Same author

Development of a Mouse Model of Uremic Cardiomyopathy: Investigating the Impact of Chronic Kidney Disease on Cardiac Function and Signaling Pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025

Same author

NYHA class and cardiopulmonary exercise capacity impact self-rated health-related quality of life in young adults after arterial switch operation for transposition of the great arteries.

International journal of cardiology. Congenital heart disease·2024

Same author

Coronary coding in dTGA pre- and post-ASO-verification and necessary corrections following adult CMR.

European heart journal. Imaging methods and practice·2024

Same author

BILATERAL CHOROIDAL DETACHMENTS FOLLOWING NOVEL CHIMERIC ANTIGEN RECEPTOR T-CELL IMMUNOTHERAPY REGIMEN.

Retinal cases & brief reports·2024

Same author

Rare Case of an Adult With Double-Chambered Left Ventricle.

CJC pediatric and congenital heart disease·2023

Area of Science:

Ophthalmology
Gene Therapy
Virology

Background:

Gene therapy utilizing viral vectors shows promise for treating ocular diseases.
Adeno-associated viruses (AAVs) offer stable, long-term gene expression and cell-type targeting in the eye.
Clinical trials are underway for ocular diseases using adenovirus and AAV vectors.

Purpose of the Study:

To compare the gene transfer efficacy and expression stability of novel AAV serotypes (AAV7, 8, 9) against established serotypes (AAV1, 2, 5).
To evaluate the potential of new AAV vectors for treating ocular conditions.

Main Methods:

In vivo comparison of gene transfer efficiency and expression stability.
Utilized AAV vectors based on serotypes 1, 2, 4, 5, 7, 8, and 9.
Assessed transduction in retinal and anterior chamber structures.

More Related Videos

Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

Published on: December 25, 2021

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Published on: August 7, 2015

Related Experiment Videos

Last Updated: Jul 7, 2026

Subconjunctival Administration of Adeno-associated Virus Vectors in Small Animal Models

Subconjunctival Administration of Adeno-associated Virus Vectors in Small Animal Models

Published on: March 16, 2022

Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

Published on: December 25, 2021

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Published on: August 7, 2015

Main Results:

AAV7 and AAV8 vectors demonstrated superior long-term transduction compared to AAV1, 2, and 5.
High levels of retinal gene transfer were achieved with established AAV vectors.
Novel serotypes showed enhanced efficacy in both retinal and anterior chamber tissues.

Conclusions:

AAV7 and AAV8 represent promising vectors for ocular gene therapy due to enhanced transduction.
These novel serotypes could improve treatment outcomes for a range of inherited and acquired ocular diseases.
Further research into AAV vector optimization is warranted for clinical applications.