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Related Experiment Video

Updated: Jul 7, 2026

Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
08:57

Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue

Published on: July 6, 2011

Depth profiling brain tissue sections with a 40 keV C60+ primary ion beam.

Emrys A Jones1, Nicholas P Lockyer, John C Vickerman

  • 1Surface Analysis Research Centre, Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester, M1 7DN, United Kingdom. emrys.jones@manchester.ac.uk

Analytical Chemistry
|February 19, 2008
PubMed
Summary

Prolonged C60(+) ion bombardment of rat brain tissue causes signal loss due to lipid migration and salt adducts. Washing the tissue and analyzing frozen samples improves chemical information retrieval for 3D SIMS mapping.

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Area of Science:

  • Biomedical analysis
  • Surface science
  • Mass spectrometry

Background:

  • Secondary Ion Mass Spectrometry (SIMS) is crucial for analyzing biological tissue.
  • Understanding ion bombardment effects is vital for accurate chemical mapping.
  • Previous studies have not fully explored C60(+) effects on complex biological samples.

Purpose of the Study:

  • To investigate the impact of prolonged C60(+) primary ion bombardment on rat brain tissue.
  • To understand the chemical changes and signal loss mechanisms during ion bombardment.
  • To evaluate the potential of high ion doses for improved 3D SIMS analysis.

Main Methods:

  • Secondary Ion Mass Spectrometry (SIMS) with C60(+) and Au(+) primary ions.
  • Analysis of rat brain tissue sections under vacuum and at room temperature.

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  • Use of ammonium formate wash to reduce salt levels.
  • Three-dimensional (3D) imaging and depth profiling in a frozen state.

    • Main Results:

    • Prolonged C60(+) bombardment led to rapid molecular signal loss.
    • Signal loss correlated with sodium and potassium adducts of phosphate and protein fragments.
    • Lipid migration to the tissue surface was observed at room temperature under vacuum.
    • Removing lipids uncovered underlying species like proteins.
    • Frozen state depth profiling revealed the extent of lipid migration.

    Conclusions:

    • Salt reduction via washing mitigates ion bombardment-induced signal artifacts.
    • Lipid migration significantly affects the observed native compound localization.
    • High ion doses, beyond the static limit, can enhance secondary ion yields for improved detection limits and lateral resolution in 3D SIMS.
    • This study provides insights into optimizing 3D SIMS for biological tissue analysis.