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Reliability of sample collection and laboratory testing for HPV detection in men
Roberto Flores1, Andrew T Abalos, Carrie M Nielson
1Division of Cancer Prevention and Control, H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, MRC-223A, Tampa, FL 33612, USA. floresr@moffitt.org <floresr@moffitt.org>
Journal of Virological Methods
|February 19, 2008
Summary
Reliable sample collection for male human papillomavirus (HPV) testing is possible using swab methods for exfoliated skin cells. Molecular testing, particularly for oncogenic HPV types, demonstrates high reliability, improving male HPV infection analysis.
Area of Science:
- Urology
- Infectious Diseases
- Molecular Diagnostics
Background:
- Male anogenital human papillomavirus (HPV) infection detection is often unreliable due to inconsistent sample collection and adequacy.
- Standardized and reliable methods are crucial for accurate HPV diagnosis and epidemiological studies in men.
Purpose of the Study:
- To assess the reliability of exfoliated skin cell sample collection from male anogenital sites for HPV detection.
- To evaluate the performance of PCR-based HPV detection and genotyping methods.
- To determine the concordance of HPV testing across different anatomical sites in males.
Main Methods:
- Collection of exfoliated skin cells from penile shaft, perianal area, anal canal, and scrotum using a swab method.
- Detection of HPV DNA using PCR-based methods and genotyping.
- Statistical analysis of concordance between paired samples from different anatomical sites using kappa statistics.
Main Results:
- The penile shaft showed the highest concordance (kappa=0.75) for HPV testing, followed by the perianal area (kappa=0.68).
- Laboratory testing reliability was highest for oncogenic HPV types (kappa=0.86) and multiple-type infections (kappa=0.84).
- The swab method for exfoliated cells is adequate for reliable molecular HPV testing.
Conclusions:
- The swab method for collecting exfoliated skin cells is suitable for male anogenital HPV testing.
- PCR-based HPV detection and genotyping are reliable, especially for oncogenic types and multiple infections.
- These findings support improved consistency and accuracy in male HPV infection surveillance and diagnosis.
